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目的 探究长链非编码RNA LINC00894对甲状腺未分化癌细胞体外凋亡、迁移和侵袭的影响及机制。方法 通过GEPIA2数据库检测LINC00894在甲状腺癌及癌旁组织中表达情况；通过KM-plotter数据库分析LINC00894表达与甲状腺癌患者总生存期相关性；RT-qPCR检测LINC00894在甲状腺未分化癌细胞中的表达。建立稳定过表达LINC00894的甲状腺未分化癌CAL-62细胞，RT-qPCR验证转染效率，分别通过流式细胞术和Transwell实验检测过表达LINC00894对甲状腺未分化癌CAL-62细胞凋亡、迁移和侵袭的影响。生物信息学分析miR-24-3p与LINC00894的潜在结合位点，并通过双荧光素酶报告基因实验验证miR-24-3p与LINC00894结合；RT-qPCR检测过表达LINC00894甲状腺未分化癌CAL-62细胞中miR-24-3p表达；ENCORI数据库分析miR-24-3p与LINC00894在甲状腺癌中表达相关性，进一步采用流式细胞术和Transwell实验检测过表达miR-24-3p对过表达LINC00894的甲状腺未分化癌CAL-62细胞凋亡、迁移和侵袭的影响。结果 LINC00894在甲状腺癌组织和甲状腺未分化癌细胞中表达下调，LINC00894低表达与甲状腺癌患者较低的总生存期相关。过表达LINC00894增加甲状腺未分化癌CAL-62细胞LINC00894表达，抑制细胞体外迁移和侵袭并促进凋亡。LINC00894与miR-24-3p结合，过表达LINC00894下调CAL-62细胞中miR-24-3p表达，miR-24-3p和LINC00894的表达在甲状腺癌中呈显著负相关。过表达miR-24-3p逆转了过表达LINC00894对CAL-62细胞体外迁移和侵袭的抑制作用及凋亡的促进。结论 LINC00894通过调控miR-24-3p抑制甲状腺未分化癌细胞的迁移和侵袭并促进凋亡。
Abstract：Objective To explore the effects and mechanisms of lncRNA LINC00894 on apoptosis, migration, and invasion of anaplastic carcinoma cancer cells in vitro.Methods The expression of LINC00894 in thyroid cancer and adjacent tissues was detected by the GEPIA2 database.The correlation between the expression of LINC00894 and the overall survival of patients with thyroid cancer was analyzed through the KM-plotter database.The expression of LINC00894 in undifferentiated thyroid cancer cells was detected by RT-qPCR.Stable overexpression of LINC00894 in thyroid anaplastic carcinoma CAL-62 cells was established.The transfection efficiency was verified by RT-qPCR.The effects of overexpressing LINC00894 on apoptosis, migration and invasion of thyroid anaplastic carcinoma CAL-62 cells were detected by flow cytometry and Transwell assay, respectively.The potential binding sites of miR-24-3p and LINC00894 were analyzed by bioinformatics, and the binding of miR-24-3p to LINC00894 was verified by the dual-luciferase reporter gene assay.The expression of miR-24-3p in CAL-62 thyroid anaplastic carcinoma cells overexpressing LINC00894 was detected by RT-qPCR.The expression correlation between miR-24-3p and LINC00894 in thyroid cancer was analyzed through the ENCORI database.Further, the effects of overexpression of miR-24-3p on apoptosis, migration and invasion of thyroid anadifferentiated carcinoma CAL-62 cells overexpressing LINC00894 were detected by flow cytometry and Transwell assay.Results The expression of LINC00894 was down-regulated in thyroid carcinoma tissues and undifferentiated thyroid carcinoma cells, and the low expression of LINC00894 was related to the lower overall survival time of thyroid carcinoma patients.Overexpression of LINC00894 increased the expression of LINC00894 in anaplastic thyroid carcinoma CAL-62 cells, inhibited migration and invasion of cells in vitro and promoted apoptosis of cells.LINC00894 binded to miR-24-3p, overexpression of LINC00894 down-regulated the expression of miR-24-3p in CAL-62 cells, and the expression of miR-24-3p and LINC00894 was significantly negatively correlated in thyroid carcinoma.Overexpression of miR-24-3p reversed the inhibitory effect of overexpression of LINC00894 on migration and invasion of CAL-62 cells in vitro and the promotion of apoptosis.Conclusion LINC00894 inhibits the migration and invasion of anaplastic thyroid carcinoma cells and promotes apoptosis by regulating miR-24-3p.
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基本信息:
DOI：10.16695/j.cnki.1006-2947.2026.01.018
中图分类号:R736.1
引用信息:
[1]张飞燕,陈继婧,郭宏鹏,等.长链非编码RNA LINC00894靶向miR-24-3p抑制甲状腺未分化癌发展[J].解剖科学进展,2026,32(01):70-74.DOI:10.16695/j.cnki.1006-2947.2026.01.018.
基金信息:
辽宁省科学技术计划重大科研项目(2022JH2/101300035)
2026-01-20
2026-01-20